增龄对人牙本质失水收缩的重要性

汪饶饶; 毛霜霜; 依赖恩·隆伯格; 德温·阿罗拉; 张东升

doi:10.3879/j.issn.1000-0887.2012.03.005

增龄对人牙本质失水收缩的重要性

doi: 10.3879/j.issn.1000-0887.2012.03.005

同济大学附属第十人民医院,上海 200072;2.上海大学力学系,上海 200444;3.上海市力学在能源工程中的应用重点实验室,上海 200072;4.马里兰大学口腔外科学院健康促进与政策研究室,巴尔的摩 21201,美国;5.马里兰大学机械学院,巴尔的摩 21201,美国;6.马里兰大学医学院牙髓学、修复学和牙科手术学系,巴尔的摩 21201,美国

基金项目: 国家自然科学基金资助项目(11172161);上海市重点学科建设基金资助项目(S30106);上海市教委重点基金资助项目(12ZZ092);口腔疾病研究国家重点实验室(四川大学)开放基金资助项目(SKLODSCU2009KF03);上海市科委基金资助项目(10410701900;11195820900;10ZR1423400);美国国立牙科和颅面研究基金资助项目（R01-DE016904）

详细信息

通讯作者:
汪饶饶(1959—), 男, 江西上饶人, 主任医师,博士(联系人.Tel:+86-21-66301726;Fax:+86-21-66301725;E-mail:raoraowang@hotmail.com).

中图分类号: Q66;R780.2
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出版历程
- 收稿日期: 2011-12-23
- 修回日期: 2012-01-13
- 刊出日期: 2012-03-15

Importance of Aging to the Dehydration Shrinkage of Human Dentin

¹Tenth People’s Hospital of Tongji University, Shanghai 200072, P.R.China;²Department of Mechanics, Shanghai University, Shanghai 200444, P.R.China;³Shanghai Key Laboratory of Mechanics in Energy Engineering, Shanghai 200072, P.R.China;⁴Department of Health Promotion and Policy Baltimore College of Dental Surgery, University of Maryland, Baltimore, MD 21201;⁵Department of Mechanical Engineering, University of Maryland Baltimore County, Baltimore, MD 21201;⁶Department of Endodontics, Prosthodontics, and Operative Dentistry Baltimore College of Dental Surgery, University of Maryland, Baltimore, MD 21201

摘要

摘要: 牙本质内的矿物质随着年龄的增长呈现增长的趋势．由于矿物质与蛋白胶原比例的改变，可能导致牙本质脱水时氢键强度和收缩程度的变化．因此，该研究的目的是确定牙本质收缩随年龄增长的量变．将磨牙牙本质的冠部制成试件，青年组：23≤age≤34，老年组：52≤age≤62，贮存在水或Hanks平衡盐溶液（HBSS）中．利用数字图像相关法(DIC)观察牙本质试件在自由空气状态下的尺寸变化过程．结果表明青年人的牙本质不管在哪个方向或存储时间上，其收缩都明显大于老年人(p<0.01)．平行于牙本质小管方向上的应变从牙髓腔侧到釉牙本质界(DEJ)侧依次递增，而垂直于牙本质小管方向的应变则依次递减．即从牙髓腔侧到DEJ牙本质收缩的各向异性增强，该现象在青年人牙本质中尤其明显．
- 牙本质 /
- DIC /
- 矿物成分 /
- 硬化症 /
- 收缩
Abstract: There was an increase in the mineral content of human dentin with aging. Due to the consequent changes in mineral to collagen ratio, this process may influence the degree of hydrogen bonding that occurs with loss of water and the extent of shrinkage as a result of dehydration. Thus, the objective of this investigation was to quantify the differences in dehydration shrinkage of human dentin with patient age. Specimens of coronal dentin were prepared from the molars of young (23≤age≤34) and old (52≤age≤62) patients and then maintained in storage solutions of water or Hanks balanced salt solution(HBSS). Dimensional changes of the dentin specimens occurring over periods of free convection were evaluated using microscopic digital image correlation(DIC). Results distinguished that the shrinkage of young dentin is significantly larger than that for old dentin, regardless of orientation and period of storage (p<0.01).Strains parallel to the tubules increased with proximity to the dentin enamel junction(DEJ) whereas the shrinkage strains in the transverse direction were largest in deep dentin (i.e. near the pulp). The degree of anisotropy in shrinkage increased from the pulp to the DEJ and was largest in young dentin.
- dentin /
- DIC /
- mineral content /
- sclerosis /
- shrinkage

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参考文献(39)

[1]	Ten Cate A R. Oral Histology: Development, Structure, and Function[M]. Seventh ed. St Louis: Mosby-Year Book Inc, 2008.
[2]	Kinney J H, Nalla R K, Pople J A, Breunig T M, Ritchie R O. Age-related transparent root dentin: mineral concentration, crystallite size, and mechanical properties[J]. Biomaterials, 2005, 26(16): 3363-3376.
[3]	Porter A E, Nalla R K, Minor A, Jinschek J R, Kisielowski C, Radmilovic V, Kinney J H, Tomsia A P, Ritchie R O. A transmission electron microscopy study of mineralization in age-induced transparent dentin[J]. Biomaterials, 2005, 26(36): 7650-7660.
[4]	Walters C, Eyre D R. Collagen crosslinks in human dentin: increasing content of hydroxypyridinium residues with age[J]. Calcif Tissue Int, 1983, 35(4/5):401-405.
[5]	Ager J W, Nalla R K, Balooch G, Kim G, Pugach M, Habelitz S, Marshall G W, Kinney J H, Ritchie R O. On the increasing fragility of human teeth with age: a deep-UV resonance Raman study[J]. J Bone Miner Res, 2006, 21(12): 1879-1887.
[6]	Arola D. Fracture and aging in dentin. Curtis R, Watson T.Dental Biomaterials: Imaging, Testing and Modeling[M]. Cambridge, UK: Woodhead Publishing, 2007.
[7]	Arola D, Bajaj D, Ivancik J, Majd H, Zhang D. Fatigue of biomaterials: hard tissues[J]. International Journal of Fatigue, 2010, 32(9):1400-1412.
[8]	Arola D, Reprogel R. Effects of aging on the mechanical behavior of human dentin[J]. Biomaterials, 2005, 26(18): 4051-4061.
[9]	Bajaj D, Sundaram N, Nazari A, Arola D. Age, dehydration and fatigue crack growth in dentin[J]. Biomaterials, 2006, 27(11): 2507-2517.
[10]	Koester K J, Ager J W, Ritchie R O. The effect of aging on crack-growth resistance and toughening mechanisms in human dentin[J]. Biomaterials, 2008, 29(10): 1318-1328.
[11]	Nazari A, Bajaj D, Zhang D, Romberg E, Arola D. On the reduction in fracture toughness of human dentin with age[J]. Journal of the Mechanical Behavior of Biomedical Materials, 2009, 2(5): 550-559.
[12]	Goodis H E, Tao L, Pashley D H. Evaporative water loss from human dentine in vitro[J]. Arch Oral Biol, 1990, 35(7): 523-527.
[13]	Matthews W G, Showman C D, Pashley D H. Air blast-induced evaporative water loss from human dentine, in vitro[J]. Arch Oral Biol, 1993, 38(6): 517-523.
[14]	Van der Graaf E R, Ten Bosch J J. Changes in dimensions and weight of human dentine after different drying procedures and during subsequent rehydration[J]. Arch Oral Biol, 1993, 38(1): 97-99.
[15]	Kinney J H, Balooch M, Marshall G, Marshall S J. Atomic-force microscopic study of dimensional changes in human dentine during drying[J]. Arch Oral Biol, 1993, 38(11): 1003-1007.
[16]	Nakaoki Y, Nikaido T, Pereira P N R, Inokoshi S, Tagami J. Dimensional changes of demineralized dentin treated with HEMA primers[J]. Dent Mater, 2000, 16(6): 441-446.
[17]	Kishen A, Rafique A. Investigations on the dynamics of water in the macrostructural dentine[J]. J Biomed Opt, 2006, 11(5): 054018.
[18]	Wood J D, Wang R Z, Weiner S, Pashley D H. Mapping of tooth deformation caused by moisture change using moiré interferometry[J]. Dent Mater, 2003, 19(3): 159-166.
[19]	Wood J D, Sobolewski P, Thakur V, Arola D, Nazari A, Tay F R, Pashley D H. Measurement of microstrains across loaded resin-dentin interfaces using microscopic moiré interferometry[J]. Dent Mater, 2008, 24(7): 859-866.
[20]	Zhang D, Mao S, Lu C, Romberg E, Arola D. Dehydration and the dynamic dimensional changes within dentin and enamel[J]. Dent Mater, 2009, 25(7): 937-945.
[21]	Carrigan P J, Morse D R, Furst M L, Sinai I H. A scanning electron microscopic evaluation of human dentinal tubules according to age and location[J]. J Endod, 1984, 10(8): 359-363.
[22]	Garberoglio R, Brnnstrm M. Scanning electron microscopic investigation of human dentinal tubules[J]. Arch Oral Biol, 1976, 21(6): 355-362.
[23]	Pashley D H. Dentin: a dynamic substrate—a review[J]. Scanning Microsc, 1989, 3(1): 161-174.
[24]	Ryou H, Amin N, Ross A, Eidelman N, Wang D H, Romberg E, Arola D. Contributions of microstructure and chemical composition to the mechanical properties of dentin[J]. J Mater Sci Mater Med, 2011, 22(5):1127-1135.
[25]	Habelitz S, Marshall G W Jr, Balooch M, Marshall S J. Nanoindentation and storage of teeth[J]. J Biomech, 2002, 35(7): 995-998.
[26]	Zhang D, Eggleton C D, Arola D. Evaluating the mechanical behavior of arterial tissue using digital image correlation[J]. Exp Mech, 2002, 42(4): 409-416.
[27]	Zhang D, Arola D. Application of digital image correlation to biological tissues[J]. J Biomed Opt, 2004, 9(4): 691-699.
[28]	Weber D F. Human dentine sclerosis: a microradiographic survey[J]. Arch Oral Biol, 1974, 19(2): 163-169.
[29]	Vasiliadis L, Darlin A I, Levers B G. The histology of sclerotic human root dentine[J]. Arch Oral Biol, 1983, 28(8): 693-700.
[30]	Nalbandian J, Gonzales F, Sognnaes R F. Sclerotic age changes in root dentin of human teeth as observed by optical, electron, and X-ray microscopy[J]. J Dent Res, 1960, 39: 598-607.
[31]	Vasiliadis L, Darling A I, Levers B G. The amount and distribution of sclerotic human root dentine[J]. Arch Oral Biol, 1983, 28(7): 645-649.
[32]	Micheletti C M. Dental histology: study of aging processes in root dentine[J]. Boll Soc Ital Biol Sper, 1998, 74(3/4): 19-28.
[33]	Van der Graaf E R, Ten Bosch J J. The uptake of water by freeze-dried human dentin sections[J]. Arch Oral Biol, 1990, 35(9): 731-739.
[34]	Shokouhinejad N, Nekoofar M H, Iravani A, Kharrazifard M J, Dummer P M. Effect of acidic environment on the push-out bond strength of mineral trioxide aggregate[J]. J Endod, 2010, 36(5): 871-874.
[35]	Prati C, Chersoni S, Mongiorgi R, Montanari G, Pashley D H. Thickness and morphology of resin-infiltrated dentin layer in young, old, and sclerotic dentin[J]. Oper Dent, 1999, 24(2): 66-72.
[36]	Tay F R, Pashley D H. Resin bonding to cervical sclerotic dentin: a review[J]. J Dent, 2004, 32(3): 173-196.
[37]	Zaslansky P, Friesem A A, Weiner S. Structure and mechanical properties of the soft zone separating bulk dentin and enamel in crowns of human teeth: insight into tooth function[J]. J Struct Biol, 2006, 153(2): 188-199.
[38]	Lopes M B, Sinhoreti M A, Gonini Júnior A, Consani S, McCabe J F. Comparative study of tubular diameter and quantity for human and bovine dentin at different depths[J]. Braz Dent J, 2009, 20(4): 279-283.
[39]	Arends J, Stokroos I, Jongebloed W G, Ruben J. The diameter of dentinal tubules in human coronal dentine after demineralization and air drying[J]. Caries Res, 1995, 29(2): 118-121.